| iVISION™ Automated Digital Imaging System |
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What is the iVISION™ System?
A: The iVISION™ Automated Digital Imaging System is a state-of-the-art, color, digital, high-resolution/high-sensitivity, automated image analysis system. It offers sophisticated hardware in an easy-to-use platform. The iVISION™ system is designed for image acquisition, archiving/retrieval, multi-parameter detection, quantitation, microvessel density and cell analysis and rare event detection. The iVISION™ Automated Imaging System is for research use only. Key features:
- Removable Slide Towers
- Data Entry and Batch Scan Capability
- Bar Code Recognition System for Protocols and Record ID
- Built-in and Modifiable Protocols for Tissue Quantitation and Rare Cell Detection
- High-Speed Scanning and Detection
- Interactive Slide Tiling and Surfing
- Measurement Tools for Line and Area Measurements
- Archiving, Quantitation, Detection, and Data/Image Management
- Auto-Pattern Detection for Multiple Samples
- TIFF and JPEG Save Options
- Customized File Maker® Database
- Report and Print Options
How many slides can the iVISION™ System process at one time?
The iVISION™ System has a capacity of 50 slides. The user has the ability to access any of the 50 slides for loading and unloading.
How long does it take to scan one slide?
This depends on the objective used and the size of the area selected. Typically, for 500 fields of view, with a 10x objective, the scan of one slide can take about +/- 5 minutes.
How long does it take to scan 50 slides?
This depends on the objective used and size of the selected area. Typically, for 500 fields of view, with a 10x objective, it can take about 5 hours to scan 50 slides.
What comes with the iVISION™ System?
- A Slide-Loading unit with a Controller
- A digital high resolution CCD camera with all the electronic component
- An Olympus microscope with optics
- An Apple PPC with all the electronic components
- A Color high resolution flat screen Monitor
- The iVISION™ software and the corresponding FileMaker® Database
How is the iVISION™ System different from competitive systems?
- The user can scan using various objectives -1.25x, 2x, 4x, 10x, 20x and even 40x
- The user can analyze slides, using scanning protocol or a different protocol
- The user can set objective and protocol differently for each slide in a batch scan
- The iVISION™ utilizes a digital high resolution CCD camera
- The user can still use the microscope and view slides through the trinoc.
- The system has an advanced customized database.
How much disk space does each scan take?
Typically the space used is about 1GB at 10x scan.
What is the general workflow of the software?
The iVISION™ System is geared towards batch scanning. The general flow starts with protocol setting and slide setup, done by the super user. The next step involves data entry and batch scan settings, done by the super user or regular user.
After which, the batch scan begins. Upon completion, the scan results are analyzed and reported upon. Finally, the report is generated through the FileMaker® database.
Does the iVISION™ have reporting capabilities?
Yes. Reports are generated through the database.
Can we print reports out?
Yes. These reports can be printed and/or saved as pdf files.
Can I see a sample of an iVISION™ report?
Yes. A sample report can be sent to you via email, fax, or mail.
What type of data is displayed on the report?
The report shows image areas selected by the user, and the corresponding protocol, intensity and population readings. It also displays patient information entered in the data entry section of the iVISION™ System.
What is the difference between the iVISION™ Digital Image Analysis and the GenoMx™ VISION Digital Image Analysis System?
The iVISION™ System is targeted to the clinical diagnosis market for tissue and rare cell slide analysis. The GenoMx™ VISION System is targeted to the drug discovery market, for tissue microarray analysis.
What protocols come with the system?
The protocols that come with the iVISION™ System are representative of nuclear, cytoplasmic and membrane staining protocols, as well as rare cell detection, multi-stain, cell analysis and tissue microarray protocols.
Are the protocols editable/customizable?
Yes. The user can edit, delete and/or add to the existing protocols.
Is the system compatible for countries using 220V?
Yes, 110V/220V power supply.
Can we save these images as TIFF or JPEG files?
Yes. Composite images and/or individual fields can be saved as high-resolution images in TIFF or JPEG format.
What kind of camera is used for the iVISION™ System?
A CCD camera with a high image detection resolution of 1030 x 1300 pixels per field of view (FOV) (~1.3 millions of pixels).
What bar code does the iVISION™ System read?
Code 128. A label printer is provided. It prints slide readable via the iVISION™ System.
What objectives come installed with the iVISION™ System?
2x, 4x, 10x, 20x, and 40x.
In terms of quantitation, what information is produced with each scan?
Generally, % intensity/intensity score and % population results are calculated for each quantitation area specified. However, more detailed information such as low, medium and highly stained percent population as well as tissue, stained, and total area values, along with a representative histogram, are also displayed.
What are the weight and dimensions of the iVISION™ System?
1101 lbs (50kg), 22 x 20 x 23 in (559 x 500 x 580 mm)
What sort of training and troubleshooting help are provided?
Training can be in-house or onsite. Technical support and field service engineers are available for troubleshooting.
What type of calibration is needed on a per use basis?
The system homes on startup and is calibrated automatically with respect to X, Y, and Z coordinates. The user performs additional system calibration (~ 5 minute process) each time the software is used.
What operating system is used?
OS 9.2.2 for the main system, OS X for the database and satellite system.
Do you plan to use OS X?
Being UNIX-based, the OSX architecture is very different from the previous OS versions. For this reason, decision to port or not to OSX will be made in the near future
Is the iVISION™ System available for Windows OS?
No.
Why Macs?
Digital imaging quality is more advanced on the Mac OS platform, allowing for more accurate analysis.
I am not familiar with Macs, does it take a while to get to know your way around?
The usage of Macs is straightforward. BioGenex representatives can train the user on the basics of Mac operation during the iVISION™ software training.
PC/Monitor specifications?
Apple dual G4 Power PC with an 80 GB application hard-drive and a 200GB hard-drive; a 750MB Zip drive or a DVD-R Super drive (Up to 9 GB/disk) with integrated 10/100/1000 Tbase Ethernet connection.
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