ZAP-70
Tonsil tissue stained with anti-Zap70 using DAB chromogen
| Clone | : | YE291 |
| Isotype | : | IgG2a |
| Immunogen | : | A synthetic peptide corresponding to residues in the C-terminus of human ZAP-70 was used as immunogen. |
| Source | : | Rabbit |
| Specificity | : | ZAP70 |
| Protocol | : | 30 min @ RT |
| Tissue type | : | FFPE |
| Control tissue | : | Tonsil Tissue |
| Pretreatment | : | EZ-AR™ 1 |
| Intended Use | : | IVD |
ZAP-70(c-term), a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation. TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70(c-term) via its tandem SH2 domains. This binding correlates with activation of ZAP-70(c-term), a critical event in T cell activation. Following TCR engagement, ZAP-70(c-term) is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Srcfamily tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70(c-term) .
