CD20 (B cell)
|Immunogen||:||Human tonsil B cells|
|Protocol||:||30 min @ RT|
This antibody reacts with a formalin-resistant intracytoplasmic epitope found in the majority of B cells which is now considered to be the CD20 antigen, a pan-B cell marker. The antibody primarily recognizes a 33 kD polypeptide B cell component and also a minor 30 kD cellular antigen. The staining pattern is consistent with pan-B reactivity, producing staining for B cells in lymphoid and peripheral blood tissue. This antibody intensely stains germinal centers and B immunoblasts in lymphoid tissue.The L26 antibody is widely regarded as the most selective B cell marker currently available. L26 has shown no cross-reactivity with nonlymphoid tissues. L26 may prove to be a useful marker for L&H variants of Reed-Sternberg cells of Hodgkin's lymphomas where reactive pattern is distinct from other Reed-Sternberg variants. This antibody stains positive for membrane and some cytoplasm for B cells.
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|CD20 (B cell)||