CD16
Lymph node stained with Anti-CD16
using AEC chromogen
| Clone | : | 2H7 |
| Isotype | : | IgG2a |
| Immunogen | : | Recombinant fusion protein corresponding to the external domain of the CD16 molecule common to both the trans-membrane form and the GPI-linked form. |
| Source | : | Mouse |
| Specificity | : | CD16 antigen |
| Protocol | : | 30 min @ RT |
| Tissue type | : | FFPE |
| Control tissue | : | LYMPH NODE OR BREAST |
| Pretreatment | : | AR-10 |
| Intended Use | : | IVD |
CD16 antigen is also known as Fc gamma receptor III and has a molecular weight of 50 to 70kD. It is a low affinity Fc receptor for complexed IgG-Fc gamma RIII, expressed on natural killer (NK) cells, granulocytes, activated macrophages and a subset of T cells expressing alpha-beta or gamma-delta T cell antigen receptors. The CD16 antigen exists both as a polypeptide-anchored form (FCgammaRIIIA or CD16A) on NK cells and monocytes/ macrophages and as a glycosylphosphatidyl-inositol (GPI)-anchored form (FcgammaRIIIB or CD16B) on neutrophils. Antibody-dependent cytotoxicity of NK cells is triggered by the engagement of CD16 with the Fc portion of IgG immunoglobulins bound to target cell-associated antigens. This antibody may be useful in the study of NK cell activity in autoimmune, neoplastic and infectious diseases. This antibody stains membrane and in the cytoplasm of positive cells.
