CD31 (Endothelial Cell)
Endothelial cells stained with Anti-
CD31 using Fast Red chromogen
| Clone | : | JC/70A |
| Isotype | : | IgG1 kappa |
| Immunogen | : | Spleen membrane from a patient with hairy cell leukemia |
| Source | : | Mouse |
| Specificity | : | CD31 antigen |
| Protocol | : | Overnight @ RT |
| Tissue type | : | FFPE |
| Control tissue | : | BREAST CARCINOMA |
| Pretreatment | : | Pepsin |
| Intended Use | : | IVD |
The localization of CD31 to regions of cell-cell contacts, and the sequence similarity to CEA, a known intercellular adhesion molecule (ICAM), strongly suggests that CD31 may function as an ICAM, possibly mediating endothelial cell-cell contacts and also promoting interactions between leukocytes and endothelial cells. Anti-CD31 monoclonal antibody JC/70A reacts with a membrane glycoprotein with an apparent size of 100 kD in endothelial cells and 130 kD in platelets. It strongly stains endothelium in normal tissue as well as benign and malignant tumor tissue. The antibody labels mega-karyocytes, platelets and occasionally plasma cells, and weakly stains mantle zone B cells, peripheral T cells and neutrophils. This antibody stains CD31 antigen in membrane and sometimes cytoplasm of endothelial and other positive cells in normal and abnormal tissues.
