Buffers and diluents are available for most applications in Immunohistochemistry and In Situ Hybridization.General buffers, such as PBS and TBS can be used for washing/rinsing of slides.
BioGenex offers several diluents for specific antibody or reagent products. The Common Antibody Diluent and Enhanced Antibody Diluents have been developed for use with all antibodies in immunohistochemistry and have been specifically optimized for use with BioGenex antibodies and reagent products. These diluents enable enhancement of signal-to-noise ratio of staining when used optimally and also help in maintaining the antibody specificity and stability. The Enhanced Antibody Diluent, in addition to all the above features, contains chemical compounds to enhance antigen-antibody interaction and affinity.
In order to achieve desired staining pattern and intensity, the titers of antibodies or concentrations of reagents may need to be optimized.