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Avidin/Biotin Blocking Kit: Certain tissues especially liver, kidney, and GI tract are rich in biotin. Use of the Avidin/Biotin Blocking kits ensures the blocking of all endogenous biotin, biotin receptors, or avidin binding sites present in the tissue. Pretreatment of tissues with avidin blocking should always be followed with biotin blocking.
Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. This Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
Power Block™ : This is a blocking reagent for reducing non-specific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods and immunogold techniques. Power Block™ reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.
Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.
Levamisole: This is used to reduce background staining of alkaline phosphatase-based detection systems by inhibiting endogenous alkaline phosphatase in most tissues. It is intended for use with detection systems that employ intestinal alkaline phosphatase as the enzyme label. Levamisole is added to an alkaline phosphatase chromogen (Fast Red or BCIP/NBT).