A classic system based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound antibody.
The immunohistochemical reaction takes place in following steps:

1. Cells or tissues are prepared and then incubated with an unlabeled primary antibody that will bind to the antigen.
2. The bound antibody is detected with a species-specific secondary antibody that will recognize a group of primaryantibodies (e.g. primary antibodies made in rabbits). The secondary antibody is labeled with multiple biotin residues.
3. The bound secondary antibody is then allowed to react with Streptavidin conjugated with an enzyme. Streptavidin binds extremely strongly and irreversibly to the biotin residues on the secondary antibody resulting in the addition of multiple enzyme to the primary antibody complex.
4. A substrate is then added and acted upon by the enzyme producing a highly visible precipitate.

• Improved staining of weak antigens
• User-friendly and extensive choice of kit formats designed for use with human, animal or rodent tissue
• Wide choice of chromogens offered

SuperSensitive™ Link-Label IHC Detection System is available in manual and automated formats.

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