Both branching phosphoramidites are derived from a unique tris(2-hydroxyethyl)cyanuric acid backbone (US Patent 5,916,750).
The Symmetric Branching Phosphoramidte (BGX-2529) generates two identical branches at the 5' terminus of an oligonucleotide during automated DNA synthesis. The number of branches added increases exponentially with each addition of the branching phosphoramidite.
The Asymmetric Branching Phosphoramidite (BGX-2539) generates two asymmetric branches at the 5' terminus of oligonucleotide during automated DNA synthesis. Multiple additions of this phosphoramidite generate "comb" type oligonucleotide structures.