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Counterstains and Mounting Media
BioGenex offers a selection of counterstains and mounting media for use in Immunohistochemistry and In Situ Hybridization. In IHC and ISH procedures, a histological or cytological specimen affixed to a microscope slide is stained with a chromogen, counterstained and mounted. The mounting medium may be used to attach a coverslip or may itself serve as a substitute for the coverslip. The choice of mounting medium depends on whether long-term ("permanent" mounting) or short-term preservation ("aqueous" mounting) is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

In addition to our standard Aqueous Mounting Medium, we offer the SuperMount® Permanent Aqueous Mounting Medium, an innovative, patented mounting medium (BioGenex's U.S. Patent No. 5,492,837) designed to preserve biological specimens for long-term storage. It is a polymer based mounting medium that does not require the use of a coverslip. SuperMount® Medium is compatible with most aqueous, organic-soluble dyes, and chromogens including:
  • AEC
  • DAB
  • Fast Red
  • New Fuchsin
  • BCIP/NBT
  • Rhodamine
  • Fluorescein
  • Texas Red
  • Phycoerythrin
  • Phycocyanin
  • Fat Stain (Oil Red O)
The refractive index of SuperMount® Medium yields greater transparency and clarity of specimens to be examined under the microscope. When applied in a thin coat and allowed to harden, SuperMount® Medium forms a highly transparent coating that permanently preserves stained tissue sections. SuperMount® Medium can be used for the mounting of all biological specimens, including stained tissue sections, cytospin preparations, and blood smears. The compatibility chart below summarizes the recommended mounting medium for different combinations of chromogens and counterstains.

The compatibility charts below summarize the recommended mounting medium for different combinations of chromogen and counterstain:

Chromogens
AECBrick RedAlcohol/Xylene Soluble
BCIP/NBTBlue-PurpleAlcohol/Xylene Insoluble
DAB BrownAlcohol/Xylene Insoluble
Fast Red RedAlcohol/Xylene Soluble
New Fuchsin RedAlcohol/Xylene Soluble
Counterstains
Mayer's Hematoxylin BlueAlcohol/Xylene Insoluble
Nuclear Fast Red RedAlcohol/Xylene Insoluble
Mounting MediaRecommendation
Aqueous Mounting MediumUse aqueous solution
Permanent Mounting mediumUse alcohol and xylene
SuperMount® Permanent Mounting MediumUse aqueous solution


Counterstains/
Stains		AECBCIP/NBTDABFast RedNew Fuchsin
HematoxylinAqueous
or
SuperMount®		Aqueous/
NR		Aqueous/
SuperMount®/
Permanent
Mounting Media		Aqueous/
SuperMount®		SuperMount®
Methyl GreenNR PermanentPermanentPermanentPermanent
Nuclear
Fast Red		NR Permanent
Mounting Media/
SuperMount®/
Aqueous		 Permanent
Mounting Media/
SuperMount®/
Aqueous		 NRNR
NR= Not recommended (may have poor color-based contrast)

CounterstainsCat. No.SizeDatasheetMSDS
Hematoxylin, Mayer's (Non-alcohol based)*HK100-5K6ml Download Download
HK100-9K250 ml Download Download
Nuclear Fast Red** HK142-7K17 ml Download Download
DAPI** CS-2010-06 6 ml Download Download
Methyl Green** HK141-7K17ml Download Download


Mounting MediaCat. No.SizeDatasheetMSDS
Aqueous Mounting MediumHK099-5K15 ml Download Download
SuperMount® Permanent Aqueous Mounting MediumHK079-5K15 ml Download Download
HK079-7K50 ml Download Download
Permanent Mounting Medium, Ready-to-UseMM-1002-1515 ml MM-1002-15 MM-1002-15
Methyl Green** HK141-7K17ml Download Download
Unless otherwise specified, all products listed here are laboratory use only
*For In Vitro Diagnostic use
**Research Use Only
   
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