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Home >>Products >> Detection Systems >> IHC Detection Systems >> iso - IHC (Mouse-on-Mouse) Detection Kits
Link-Label (Biotin based)
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IHC for Rodent Tissue
Iso-IHC (mouse-on-mouse)
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iso - IHC (Mouse-on-Mouse) Detection Kits
The iso-IHC Detection Kits allow the detection of mouse antibodies on mouse tissues. The procedure suppresses the strong background caused by the cross-reactivity of the anti-mouse secondary and produces exceptionally high signal-to-noise ratios with strong staining intensities and no background. The method is based on the pre-labeling of mouse antibodies with an exclusive Biotin-labeling Reagent. This reagent biotinylates only mouse primary antibodies, even in the presence of contaminating proteins. Excess biotinylation reagent is blocked with the proprietary Mouse Block Reagent. The Biotinylated primary antibody mixture is added directly to the cell or tissues. The bound Biotinylated antibody is detected by the addition of a Streptavidin-conjugate that attaches strongly to the biotin residues on the primary antibody. If a Streptavidin-Enzyme conjugate was used, a chromogen/substrate is added and the resultant colored precipitate visualized by light microscopy.

The iso-IHC kits come in two formats:
  • Complete kit - contains all components required to perform staining
  • Core Kit - offers the user the freedom to choose chromogen of choice

Complete Kits
The Complete iso-IHC Kits come in 60-slide and 250-slide format.

Chromogen Cat. No Size Datasheet MSDS
AECHC-3019-05* 60 slides Download Download
DABHC-3019-06* 60 slides Download Download
DABHC-3119-06* 250 slides Download Download
* Research Use Only

These kits contain the following components:

Contents Ready-to-Use Kits
(60 slides)		 Ready-to-Use Kits
(250 slides)
Peroxide Block6 ml RTU25 ml RTU
Power Block™ Reagent0.6 ml RTU25 ml RTU
Labeling Reagent0.6 ml RTU2.5 10X
MouseBlock Reagent0.6 ml RTU2.5 ml RTU
Streptavidin-Conjugate6 ml RTU25 ml RTU
Chromogen6 ml RTU25 ml RTU
Counterstain6 ml RTU-----
Mounting Medium15 ml RTU-----
Wash Buffer 20 Tablets50 Tablets


Core Kit
The iso-IHC Kit comes in 60-slide format.   Catalog # ZA010-60K

Contents Ready-to-Use
(60 slides)
Power Block™ Reagent0.6 ml RTU
Labeling Reagent0.6 ml RTU
MouseBlock Reagent0.6 ml RTU
Streptavidin-Alkaline PhosphataseConjugate6 ml RTU
Activation Buffer6 ml RTU
PBS Tablets20 Tablets


iso-IHC Core Kit Accessories
The following accessories may be used with the iso-IHC Core Kit:

Accessory Cat. No Size Data Sheet MSDS
BCIP/NBT ChromogenCH-0801-06 0.6 ml RTU Download Download
Fast Red Substrate PackHK182-5K 500 slides Download Download
Naphthol phosphate substrate (for use with Fast Red tablets)HK180-7K 15 ml Download Download
Fast Red tabletsHK181-7K 11 tablets Download Download
SuperMount™ Permanent Aqueous Mounting MediumHK079-5K 15 tablets Download Download
Mayer's HematoxylinHK100-5K 6 ml Download Download
HK100-9K 250 ml Download Download
   
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