Antigen retrieval is an effective method of unmasking antigenic epitopes on the surface of formalin-fixed paraffin-embedded (FFPE) tissue sections. The antigen retrieval technique breaks the methylene bridges between epitopes and unrelated proteins to expose antigenic sites for antibody binding (1). BioGenex Laboratories Inc. invented the reverse epitope masking method in 1991 (2), with this assay now being routinely practiced in laboratories throughout the world. For in-depth article on Antigen Retrieval click here.
EZ-AR2 Elegance Antigen Retrieval Solution:
BioGenex EZ-AR2 Elegance is the first universal one-step solution for preparation of formalin-fixed paraffin-embedded (FFPE) tissue sections for IHC staining. The high throughput system is designed to perform de-wax, rehydration, and antigen retrieval all in one simple step. EZ-AR2 Elegance buffer together with EZ-Retriever® processes about 100 slides in less than 30 minutes. Efficient antigen retrieval allows higher dilution of antibodies and shortens incubation times for many antibodies – cost-effective and saves time!